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Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and <t>S100</t> (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.
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Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and <t>S100</t> (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.
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Image Search Results


Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and S100 (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet: Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and S100 (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.

Article Snippet: Immunohistochemical localization for E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), was made as previously described.

Techniques: Expressing

Effect of BITC on S100 expression in an orthotopic model of ATC (A, B, D, and E) The immunohistochemical assay revealed a significant increase of S100 expression in the ATC group (B) compared to Sham mice (A); this expression was reduced following treatment with BITC at doses of 5 mg/kg (C),10 mg/kg (D) and 30 mg/kg (E) compared to ATC group (B) (see score F). The data are representative of at least three independent experiments. The sections were observed and photographed at 20× magnification. Values are means ± SEM. One-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons. ∗∗∗ p < 0.001 vs. Sham; # p < 0.05 vs. ATC.

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet: Effect of BITC on S100 expression in an orthotopic model of ATC (A, B, D, and E) The immunohistochemical assay revealed a significant increase of S100 expression in the ATC group (B) compared to Sham mice (A); this expression was reduced following treatment with BITC at doses of 5 mg/kg (C),10 mg/kg (D) and 30 mg/kg (E) compared to ATC group (B) (see score F). The data are representative of at least three independent experiments. The sections were observed and photographed at 20× magnification. Values are means ± SEM. One-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons. ∗∗∗ p < 0.001 vs. Sham; # p < 0.05 vs. ATC.

Article Snippet: Immunohistochemical localization for E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), was made as previously described.

Techniques: Expressing, Immunohistochemical staining

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet:

Article Snippet: Immunohistochemical localization for E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), was made as previously described.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-S100 , Santa Cruz Biotechnology , sc-393919 lot# C1120; RRID: AB_2910203.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and S100 (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet: Effect of BITC on EMT markers expression in an orthotopic model of ATC (A) The blots revealed a significant modulation of EMT markers expression following BITC treatment. (B–D) BITC at the doses of 5 mg/kg, 10 mg/kg and 30 mg/kg was able to significantly reduce the expression of N-cadherin (B) and S100 (D) # p < 0.05, ## p < 0.01 vs. ATC; at the same time 10 mg/kg and 30 mg/kg BITC treatment significantly increased the expression of E-cadherin (C) compared to ATC group; # p < 0.05 vs. ATC; ## p < 0.01 vs. ATC. Values are means ± SEM. We used one-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons.

Article Snippet: Membranes were blocked with 5% (w/v) dried nonfat milk in buffered saline (PM) for 45 min at room temperature and subsequently probed with specific antibodies: anti-BID (1: 500; sc-11423; Santa Cruz Biotechnology), anti-BCL-2 (1: 500; sc-7382; Santa Cruz Biotechnology), anti-BAD (1:500; sc-8044; Santa Cruz Biotechnology), anti-pmTOR (1:500; S2448; Cell Signaling), anti-p62 (1:500; 5114S; Cell Signaling), anti-PTEN (1:500; sc-7974; Santa Cruz Biotechnology) and anti-SOS1 (1:500; sc-17793; Santa Cruz Biotechnology), anti-E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), anti-N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and anti-S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), in 1× PBS, 5% w/v dried nonfat milk and 0.1% Tween-20 (PMT) at 4°C overnight.

Techniques: Expressing

Effect of BITC on S100 expression in an orthotopic model of ATC (A, B, D, and E) The immunohistochemical assay revealed a significant increase of S100 expression in the ATC group (B) compared to Sham mice (A); this expression was reduced following treatment with BITC at doses of 5 mg/kg (C),10 mg/kg (D) and 30 mg/kg (E) compared to ATC group (B) (see score F). The data are representative of at least three independent experiments. The sections were observed and photographed at 20× magnification. Values are means ± SEM. One-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons. ∗∗∗ p < 0.001 vs. Sham; # p < 0.05 vs. ATC.

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet: Effect of BITC on S100 expression in an orthotopic model of ATC (A, B, D, and E) The immunohistochemical assay revealed a significant increase of S100 expression in the ATC group (B) compared to Sham mice (A); this expression was reduced following treatment with BITC at doses of 5 mg/kg (C),10 mg/kg (D) and 30 mg/kg (E) compared to ATC group (B) (see score F). The data are representative of at least three independent experiments. The sections were observed and photographed at 20× magnification. Values are means ± SEM. One-way ANOVA test followed by Bonferroni post hoc test for multiple comparisons. ∗∗∗ p < 0.001 vs. Sham; # p < 0.05 vs. ATC.

Article Snippet: Membranes were blocked with 5% (w/v) dried nonfat milk in buffered saline (PM) for 45 min at room temperature and subsequently probed with specific antibodies: anti-BID (1: 500; sc-11423; Santa Cruz Biotechnology), anti-BCL-2 (1: 500; sc-7382; Santa Cruz Biotechnology), anti-BAD (1:500; sc-8044; Santa Cruz Biotechnology), anti-pmTOR (1:500; S2448; Cell Signaling), anti-p62 (1:500; 5114S; Cell Signaling), anti-PTEN (1:500; sc-7974; Santa Cruz Biotechnology) and anti-SOS1 (1:500; sc-17793; Santa Cruz Biotechnology), anti-E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), anti-N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and anti-S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), in 1× PBS, 5% w/v dried nonfat milk and 0.1% Tween-20 (PMT) at 4°C overnight.

Techniques: Expressing, Immunohistochemical staining

Journal: iScience

Article Title: Benzyl isothiocyanate suppresses development of thyroid carcinoma by regulating both autophagy and apoptosis pathway

doi: 10.1016/j.isci.2024.110796

Figure Lengend Snippet:

Article Snippet: Membranes were blocked with 5% (w/v) dried nonfat milk in buffered saline (PM) for 45 min at room temperature and subsequently probed with specific antibodies: anti-BID (1: 500; sc-11423; Santa Cruz Biotechnology), anti-BCL-2 (1: 500; sc-7382; Santa Cruz Biotechnology), anti-BAD (1:500; sc-8044; Santa Cruz Biotechnology), anti-pmTOR (1:500; S2448; Cell Signaling), anti-p62 (1:500; 5114S; Cell Signaling), anti-PTEN (1:500; sc-7974; Santa Cruz Biotechnology) and anti-SOS1 (1:500; sc-17793; Santa Cruz Biotechnology), anti-E-Cadherin (1:100; sc-8426 Santa Cruz Biotechnology, Dallas, TX), anti-N-Cadherin (1:100; sc-8424 Santa Cruz Biotechnology, Dallas, TX), and anti-S100 antibodies (1:100; sc-393919 Santa Cruz Biotechnology, Dallas, TX), in 1× PBS, 5% w/v dried nonfat milk and 0.1% Tween-20 (PMT) at 4°C overnight.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software